THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

SAPCALIU, A.; BUTU, Alina; CONDUR, D.; RADOI, I.

THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA
A. SAPCALIU^*, Alina BUŢU^**, D. CONDUR^***, I. RADOI^****
^* Institute for Beekeeping Research and Development, Bucharest
^** National Institute of Research and Development for Biological Sciences, Bucharest
^*** Spiru Haret University Bucharest
^**** USAMV Bucharest

Abstract. Although biochemical analyses of the blood were and are still used for the routine diagnosis and especially for the metabolic survey in farm animals, such analyses, may be applied for the honeybee hemolymph (as a paraclinic examination). The aim of this experimental study was to investigate and to determine the reference values of the main biochemical parameters in the hemolymph of the healthy honeybees of Apis mellifera carpathica. The honeybee samples were collected in order to analyze the health condition of the respective colony. All the samples coming from sick colonies were removed, only healthy adult honeybees coming from strong colonies were kept and used for hemolymph collection. By special methods, samples of hemolymph (300 μl/sample) collected from about 50 individuals, were analyzed both during the active season (spring/summer) and the inactive season (autumn/winter). The study was carried out on 50 samples of undiluted hemolymph taken from a total number of about 2,500 honeybees. The test was carried out after the collection and processing of the samples using the SPOTCHEM EZ_SP4430, equipment with dry kits, the slides technique, respectively.